Reporter

Part:BBa_K3026004:Design

Designed by: Francois D Rouleau   Group: iGEM19_ULaval   (2019-09-08)


ToeHold_DNA_SecA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 168


Design Notes

ToeHold were designed with sequence constraints according to the engineered design from Green et al. 2009. sfGFP was chosen according to its expression profile in cell-free media containing T7 polymerase, and according to its excitation and emission spectra.


Source

Synthetic construct. Switch sequence comes from E. coli BL21 DE3 genomic DNA. sfGFP sequence comes from part BBa_K1321337. Other ToeHold sequence come from Green et all 2009 Supp. Data switch #1

References